- One of the key responsibilities of chemical pigment manufacturers is to develop pigments that are safe, non-toxic, and environmentally friendly. Today, there is a growing demand for greener and more sustainable products, and chemical pigment manufacturers need to keep up with these trends by using eco-friendly raw materials and production processes. This includes reducing the use of harmful chemicals and promoting recycling and waste management practices.
Because of health risks, France banned titanium dioxide as a food additive in 2020. Two years later the European Union also banned titanium dioxide as a food additive.
- Potential Risks of Using Titanium Dioxide
Gravimetric analysis is a method of quantitative chemical analysis in which a substance is converted into a stable compound of known composition, and the mass of this compound is then measured. This technique offers a high level of precision and is particularly useful in determining the concentration of materials within complex mixtures. The gravimetric determination of titanium dioxide typically involves a series of well-defined steps, which include precipitation, filtration, washing, drying, and weighing.
The toxicity of P25TiO2NPs was evaluated in both prokaryotic (Fig. 3) and eukaryotic cells (Fig. 4). The XTT assay was chosen to measure the cell viability in bacterial cultures of MSSA, a normal skin microbiota microorganism. The reduction in the viability of samples with bare NPs is notorious, possibly due to the described ROS production from the interaction of P25TiO2NPs with light [37]. This effect seems to be avoided when they are functionalized with vitamin B2. Also, the most concentrated vitaminB2@P25TiO2NPs sample (0.2 mg/mL) shows up to 60% more absorbance after 6 h compared to the bare NPs (due to normal cell replication). This may indicate that the antioxidant effect of the vitamin B2 coating is greater than the oxidation damage produced by the NPs. This protective capacity could be attributed to the glutathione redox cycle and the conversion of reduced riboflavin to its oxidized form [38]. Values of cell viability greater than 100% are not rare and could be understood because the XTT assay actually measure metabolic activity when reducing the tetrazole to formazan. It is usually assumed that conversion is dependent on the number of viable cells, but it could also be related to an expected increased enzymatic activity when cells are exposed to small doses of some new substance. Further analysis showed that this effect was not the only one responsible for better cell viability of vitaminB@P25TiO2NPs treated samples.


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Genotoxicity and cytotoxicity
Lithopone 30% CAS No. 1345-05-7 / Nature and stability
For a mini-review published in the journal Particle and Fibre Technology in 2021, scientists wanted to evaluate whether Ti02 particles contributed to the development and/or exacerbation of irritable bowel disease, and whether they altered the four elements of intestinal barrier function: the intestinal microbiota, the immune system, the mucus layer, and the epithelium. The breakdown of these four elements can contribute to autoimmune, neurological, inflammatory, infectious, and metabolic diseases. Following their review, the researchers concluded: “Data indicate that TiO2 is able to alter the four compartments of IBF and to induce a low-grade intestinal inflammation associated or not with pre-neoplastic lesions.”
When E171 isn’t combined with other ingredients and administered in water, some studies suggest that under these artificial conditions, E171 may be processed differently in the body resulting in some biological changes in experimental animals that are poorly understood.